We performed a comprehensive two-hybrid analysis of the budding yeast proteome. We cloned almost all of the full-length yeast ORFs individually as a DNA-binding domain fusion (bait) in a MATa strain and an activation domain fusion in a MATa strain. These clones were subdivided into pools, each containing up to 96 clones. Following the systematic mating of these pools, survivors of two-hybrid selection were subjected to sequence-tagging of bait and prey to obtain Interaction Sequence Tags (ISTs).
While "full data" contain all of the 4,549 interactions that we detected, "core data" include 841 interactions with more than 3 IST hits. Both tables contain gene name, ORF name, brief description of each bait and prey, information on their co-occurrence in literature, and other lines of evidence for their biological relevance, from which you can learn whether the interaction has been reported by others, whether synthetic lethality is reported between the two genes, whether the two genes are co-expressed, whether the two proteins have alternative interaction paths, whether similar interactions are identified in other species and how many IST hits the interaction has. All of these feature would help you evaluate the biological relevance of each interaction to build your own model for interaction networks. A viewer software for model construction is developed by INTEC Web & Genome Informatics Corporation.
We also attached a table containing all of the available interaction
data on yeast poteins.
Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K.,
Kuhara, S. & Sakaki, Y.
"Toward a protein-protein interaction map of the budding yeast: a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"
Proc. Natl. Acad. Sci. USA 97, 1143-1147, 2000.
Chiba, T., Ozawa, R., Yoshida, M., Hattori, M. & Sakaki, Y.
"A comprehensive two-hybrid analysis to explore the yeast protein interactome"
Proc. Natl. Acad. Sci. USA 98, 4569-4574, 2001.
Full data (4,549 interactions)
Excel format fileCore data (841 interactions with more than 3 IST hits)Compressed File (gzip format. 300K byte - Recomended)HTML file
Uncompressed file (1.3M byte)Huge file. (11.2M byte )Text file
Excel fileCompressed File (gzip format. 1K byte - Recomended)HTML file
Uncompressed file (274K byte)Huge file. (2.0M byte)Text file
You can easily see the results of our comprehensive two-hybrid analysis and previously reported protein-protein interactions using:
Workbench System for Support of Gene Regulatory Network Construction